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University of Rochester Laboratory Technician III (S) - 218756 in Rochester, New York

Laboratory Technician III (S) Job ID 218756

Location

School of Medicine & Dentistry

Full/Part Time Full-Time

Favorite Job

Regular/Temporary Regular

Opening

Full Time 40 hours Grade 077 Ctr Musculoskeletal Research

Schedule

8 AM-4:30 PM

Responsibilities

Position Summary:

The Center for Musculoskeletal Research is seeking a Full-Time Lab Tech III to work in a basic science laboratory, to conduct research relating to diagnosis and therapy for orthopaedic infections, particularly those caused by Staphylococcus aureus. The individual will be supervised by Dr. John L. Daiss. Training in specific laboratory techniques and methods will be provided by Dr. Daiss and other laboratory staff members. This individual will conduct experiments, working with daily supervision. Over time, the individual will develop more independence in planning and conducting experiments, and will be expected to plan and perform many experiments on an independent basis. The position will require some background in cell and molecular biology and/or immunology and will require excellent documentation skills. The following equipment is utilized: Laminar flow cabinet, electrophoresis equipment (gel boxes, power supplies), inverted light and fluorescence microscopes, centrifuges (mini-, regular and ultra), cell culture equipment and freezers (-20, -80, LN2), protein purification systems, animal imaging systems, biophysical analytical instruments. Use of other equipment may also be required, in which case special training will be provided.

Responsibilities:

  • Maintain hybridoma cell lines including harvesting and purifying monoclonal antibodies. Prepare frozen stocks of hybridomas for storage. Characterize and document information of the monoclonal antibodies obtained.

  • Maintain bacterial strains for various experiments. Most will be Staphylococcus aureus including numerous clinical isolates of methicillin- and vancomycin-resistant S. aureus (MRSA and VRSA). Characterization of strains for key clinically relevant parameters.

  • Production of recombinant antigen(s) including purification and detailed characterization.

  • Animal studies using mice to test efficacy of passive immunotherapy of S. aureus infection with monoclonal antibodies. Will involve special training.

  • Immunoassays of host immune responses to S. aureus infections in both animal models and human specimens.

  • Characterization of monoclonal antibodies and their interactions with recombinant and native antigens using polyacrylamide gel electrophoresis, isoelectric focusing, surface plasmon resonance and others.

  • Perform general laboratory duties such as preparing reagents and re-stocking supplies, and/or propagating important cells and cell lines.

  • Participate in scientific seminars. Prepare and present data, at laboratory meetings and at local/national research gatherings.

Qualifications:

Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience.

Preferred Qualifications

B.S. degree in a related field. Previous instruction in theoretical aspects of cell and molecular biology, or immunology, is highly desirable. Extensive prior research experience is not necessary, although it may be helpful. The successful candidate should have a strong interest in future graduate studies.

How To Apply

All applicants must apply online.

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