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University of Rochester Laboratory Technician III (S) - 223557 in Rochester, New York

Laboratory Technician III (S)

Job ID

223557

Location

School of Medicine & Dentistry

Full/Part Time

Full-Time

Favorite Job

Regular/Temporary

Regular

Opening

Full Time 40 hours Grade 077 Pediatrics M&D Neonatology

Responsibilities

Position Summary:

This position requires active participation in research laboratory activities. This individual will participate in basic science laboratory procedures including routine experiments using both cell culture and transgenic mouse models of erythropoiesis. Research will require experience with cell culture, mouse breeding/genotyping, FACS analyses, polymerase chain reaction, cloning techniques, tissue culture procedures, and fluorescent antibody detection. This position will involve handling mice and analyzing erythropoiesis in both embryos and adult animals.

The technician must be able to work in a collaborative environment and must be comfortable working with mice. Results from these experiments will be used in both publications and grant proposals.

NOTE: This document describes typical duties and responsibilities and is not intended to limit management from assigning other work as required.

Major Responsibilities:

Animal Procedures :

  • Maintain and organize mouse colonies

  • Perform genotyping

  • Assist in analyzing the erythroid phenotype of transgenic mice and embryos (training provided)

  • Generate DNA, RNA, and protein from sorted populations of cells.

Cell Culture :

  • Culture mouse and human erythroblasts, including monitoring of cultures using benzidine and trypan counts

  • Assist in shRNA knockdown by running qPCR or Western blots

  • Assist in Crisp genome editing experiments through genotyping

  • Analyze cultures using flow cytometric analyses

Research Laboratory Procedures:

  • Organizing the work stations with the necessary supplies, including assembling any necessary plastic ware

  • Cell staining and counting for flow cytometry experiments

  • Routine cleaning of instrumentation and work area per protocol

  • Restocking of work station supplies

  • Performing routine laboratory analyses including (but not limited to) RT-PCR, western blot, immunofluorescence, cell culture, and microscopy.

Participate in lab meetings and journal clubs. Keeping current in the field through pertinent seminars and literature. Laboratory management procedures such as ordering supplies, adhering to regulatory policies, maintaining certifications and training, and troubleshooting workflow problems.

Help to train and assist new student employees, potentially in research lab procedures, data entry and checking, and stocking supplies.

Required Competencies:

Demonstrates skill in communication both verbally and in writing. Demonstrate efficiency in prioritizing assignments, skilled in proactively resolving problems, and recommending and implementing continuous quality improvements. Prior experience with molecular biology methods and experience in handling biospecimens is required.

Supervision:

The position reports directly to the P.I., with support from senior laboratory staff members, and must be capable of working both independently, using sound judgment and according to University standards for good laboratory and clinical research practices, and as part of a team. The individual will be responsible for training and monitoring of additional laboratory personnel in the methods for which they have been trained.

Supervisory Responsibilities:

None other than training and monitoring of laboratory personnel as noted above.

Equipment:

Commonly used laboratory equipment will include but not limited to PCR/qPCR machines, Dissection/ Biosafety cabinets/hoods, cell culture hood/incubators, microscopes (Light and fluorescent), camera, slide stainer, gel imager/maker, autoclave, Diagenode Biorupter, centrifuges, flow cytometer, freezers, pipettors, oxygen chambers/ analyzers, desktop computer with software (Microsoft Office), printers, copier, and telephone.

Biological Agents/Reagents:

Will include working with viruses, potentially including but not limited to lenivirus and retrovirus for shRNA experiments.

Training / Certification Expectations:

Maintains all relevant animal training for mice per University protocols and guidelines.

Requirements:

Associate’s degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience. BS/BA in biological/physical science or equivalent experience is preferred. Advanced education and experience is valued. A minimum of 1-year experience in laboratory science desired. Individuals with experience in handling mice and/or cytometry are encouraged to apply. Knowledge of University policy and procedures is desirable. Must have reliable/efficient transportation.

Candidate should be highly organized and detail/documentation oriented. Strong interpersonal skills, including verbal and written communication are essential.

Working Conditiions:

  • Laboratory research duties will require working in a modern biomedical research facility, with a large interdependent, highly collaborative research staff, in the vicinity of properly contained chemical, biological materials.

  • Duties will necessarily involve working with mice. This will require fine motor manipulations as well as familiarity with biosafety techniques.

  • All procedures and facilities conform to federal, local and institutional safety and ethical guidelines.

Shift: Primarily Day Shift; Schedule varies based on Laboratory needs. May involve some weekend and evening hours within 40 hour work week.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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