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University of Rochester Laboratory Technician III (S) - 233353 in Rochester, New York

Laboratory Technician III (S) Job ID 233353

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 077 Environmental Medicine

Schedule

8:30 AM-5 PM

Responsibilities

POSITION SUMMARY:

The laboratory’s research focuses on understanding the mechanisms and treatments of chronic obstructive pulmonary disease/emphysema, asthma, and pulmonary fibrosis. The candidate will be responsible for record-keeping; submitting and modifying animal protocols ; working closely with Vivarium and DLAM staff; and exposing mice to cigarette smoke inhalation. The position will also be responsible for carrying out the laboratory experiments in vitro cell culture and in vivo (cigarette smoke, e-cigarette and waterpipe/hookah exposure to mice and procedures); assisting several technicians and postdoctoral fellows in the laboratory; and managing all aspects of animal protocols and laboratory work from data generation to data analysis. The execution of experiments will require proficiency in cell and molecular biology techniques including, but not limited to: RNA isolation, quantitative PCR, DNA cloning and expression, as well as cell culture, SDS-PAGE, Western blotting, immunocytochemistry and immunohistochemistry.

SUPERVISION AND DIRECTION EXERCISED:

None.

MACHINES AND EQUIPMENT USED:

Laboratory equipment, tools and apparatus; cigarette smoking machine; computer and/or peripheral equipment.

TYPICAL DUTIES:

Essential Tasks

30% Plans and conducts standardized technical procedures and lab experiments. Techniques to be performed: Western blotting, immunostaining, lung function measurements experiments, ELISA, Northern blotting, ribonuclease protection assays, tissue section staining, Diff-Quick staining, immunohistochemistry, in situ hybridization. RNA isolation and DNA isolation, ChIP assay, EMSA (gel shift assay), RT-PCR, restriction enzyme digests, plasmid purification and extraction, analyzing results and record keeping. Performs new experiments and assays in cells and tissues. Engages in experimental design and independent execution of above methods/protocols/assays. Assists and trains undergraduate students, graduate students and postdoctoral fellows to learn and perform assays, routine techniques/methods, and procedures for their experiments. Trains new laboratory personnel in fundamental lab techniques. Works with students and fellows/coordinators on basic and clinical research projects in collecting biological samples and performs experiments and assays (cell biology, biochemical and molecular biology techniques) in cells and biological tissues.

25% Maintains the colonies of transgenic and knockout mice; breeding of mice, record-keeping, animal protocols submissions/modifications, working closely with Vivarium and DLAM staffs, cigarette smoke inhalation exposure to mice and carrying out the laboratory experiments in vitro and in vivo (cigarette smoke exposure to mice) exposing cigarette smoke to mice, preparing cigarette smoke extracts. Animal Care: animal cigarette smoke exposures and inhalation (oral, s.c., i.m., i.v., or i.p.), lung and heart removal, dissection and tissue preparation. Maintains UCAR paperwork/protocols, orders new rodents, checks animals daily, oversight of DLAM for their maintenance of one animal room. Keeps track of various training and annual updates for all laboratory personnel so that the labs are in compliance with these offices. Ensures all other laboratory personnel are up-to-date on required laboratory and animal usage training. Keeps track of Institutional Biosafety Committee approvals, EH&S, IRB/RSRB and DLAM/UCAR training and annual updates for laboratory personnel and compliance. Coordinates clinical studies with clinical research center and the lab, assists in subjects recruitment and collection of biological samples.

25% Orders equipment and laboratory supplies, sets up experiments, cleans and performs routine maintenance on equipment. Places orders for animals and lab supplies, schedules maintenance for lab instruments, oversight and routine biochemical and molecular biological assays. Files order forms, keeps schedule for equipment maintenance, maintenance of computer records for lab and equipment, maintenance of chemical inventory and autoclave items, interacts with sales representatives, some cleaning and storing of glassware, oversight and record keeping for hazardous waste disposal. Plans and conducts standardized technical procedures and lab experiments. Sets up and operates equipment or instrumentation. Oversees and maintains laboratory. Serves as liaison of laboratory to Departmental and University administration. Assists in administrative aspects for submission of publications, grant proposals, and progress reports.

10% Maintains laboratory supplies and inventories (chemicals, animals and equipment). Manages and maintains laboratory inventory and address needs for instrument repair.

5% Participate in lab meetings and seminars. Assist the Principal Investigator with preparing figures, data sets and written text for grants and publications.

5% Other job duties as assigned.

QUALIFICATIONS:

*Education/Experience (minimum required):

  • Associate’s degree in biological sciences (Bachelor’s degree in biological or Life Sciences preferred), plus 2-3 years specialized experience in related field; or equivalent combination of education and experience.

  • E xperience in animal (mouse) work and cell culture work.

  • Experience in routine biochemical and molecular biological techniques, and practical laboratory and animal experience are desirable.

  • Must be willing to work with human biological samples, handling animals (mice) and animal tissues.

  • Ability to communicate clearly, including inwriting is essential.

  • An ability to work independently and as a member of a team.

  • Good organizational skills, ability to use good judgment, and demonstrate attention to detail are necessary.

  • Must be able to work some evenings and weekends to conduct experiments.

  • Interest in and willingness to learn new techniques are essential job components.

    • License/Certification Required: None

    Will provide training for lab biosafety, vivarium, and human subjects recruitments and biological samples studies.

    How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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