Laboratory Technician III (S)
This is a Technical position within the Loiselle Laboratory in the Center for Musculoskeletal Research.
The job consists of laboratory work using complex genetic mouse models, cell culture systems and molecular biology techniques to understand tendon biology and healing. The successful candidate will have some experience with molecular biology or related techniques, outstanding organizational skills, careful attention to detail and strong communication skills. This position involves mouse colony management, mouse recovery surgery (flexor tendon repair), tissue harvest and sample processing (preparation of mouse tissues for histology and biomechanical analyses), molecular biology techniques (PCR, gel electrophoresis, Western blotting, etc.), cell culture skills (mammalian cell culture, transfection), and data analysis/interpretation. , Hours of the position are 8:00am – 5:00pm Monday through Friday (flexible), work on limited weekends may be required. More information on the Loiselle Lab is available at: http://www.urmc.rochester.edu/musculoskeletal-research/research-labs/loiselle-lab/index.cfm
Breed, maintain, and genotype mice for multiple projects within the lab and keep detailed electronic inventory records of the mouse colonies. (20% effort)
Perform surgical procedures such as the flexor tendon injury and repair model for the support of ongoing research projects. (20% effort)
Dissection of mouse tendon and hindlimb tissues, sectioning of paraffin or frozen tissues, histological staining, histomorphometry, immunohistochemistry, and in situ hybridization of samples for ongoing research projects. (20% effort)
Dissection of mouse tendon and hindlimb tissues for biomechanical testing for ongoing research projects. (20% effort)
Isolation and analysis of RNA and protein from cell cultures and tendon repairs via real time quantitative PCR and Western blotting, respectively. (10%)
Maintenance of a detailed lab notebook with experimental detail, methods, data compilation and data analysis and interpretation included. (10%)
Associate's degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience.
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How To Apply
All applicants must apply online.
EOE Minorities/Females/Protected Veterans/Disabled
Pay Range: $ 16.49 - $ 23.08 Hourly
The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job’s compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.
Location: School of Medicine & Dentistry
Full/Part Time: Full-Time
Opening: Full Time 40 hours Grade 077
Schedule: 8 AM-5 PM