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University of Rochester Laboratory Technician IV (S) - 224382 in Rochester, New York

Laboratory Technician IV (S)

Job ID

224382

Location

School of Medicine & Dentistry

Full/Part Time

Full-Time

Favorite Job

Regular/Temporary

Regular

Opening

Full Time 40 hours Grade 079 Med M&D-Aab CardiovascResInst

Schedule

8 AM-5 PM

Responsibilities

GENERAL PURPOSE:

With minimal supervision, the Tech IV will be responsible for carrying out experiments related to ongoing studies of cardiovascular function for 5 NIH funded projects The results of these experiments will be analyzed and used to generate manuscripts for scientific journals and grant proposals.

SUPERVISION:

This position does not supervise others but the Tech IV will help orient and train other lab members.

SPECIFIC RESPONSIBILITIES:

Responsibilities will include independent performance of genotyping to analyze genetic differences between mouse strains related to ongoing studies of cardiovascular function using some theoretical knowledge to interpret data and troubleshoot experiments. Techniques include sample purification, PCR and gel electrophoresis. This position will assist with CVRI and lab orientation, training and education of new lab members, including students, in experimental technique and will contribute to animal colony maintenance and experimentation.

KNOWLEDGE AND EXPERIENCE USING THE FOLLOWING EQUIPMENT/PROGRAMS:

Spectrophotometer; water baths; various centrifuges; pipettes; thermocycler; water purification system; Biosafety cabinet; incubators; gel apparatus; pH meter; analytical balances; electrophoresis equipment; liquid nitrogen tank for cell storage; -80C freezer; digital imaging equipment. Computer skills necessary for data collection include Excel, Word, and FileMaker Pro.

RESPONSIBILITIES:

25% Perform sample purification; perform genotyping by PCR, perform gel electrophoresis. On a daily basis, enable and organize samples and reagents for genotyping purposes. Record and report data using established methods and discuss results with supervisor.

25% Independently perform and distribute routine HUVECs, MASMs, and lung endothelial cell isolations.

10% Oversee supply inventory of required reagents; update supply inventory by completing standardized paperwork to replenish supplies, record items received, route packing slips and follow-up with purchasing clerk concerning errors and items not received. Using FileMaker, generate new orders.

10% Facilitate mouse tissue specimen processing and inventory to meet the goals of the research project.

10% Troubleshoot protocols to improve outcomes; suggest modification in method and technique as needed. Perform experiments and maintain support to faculty member for grant period. Maintain laboratory notebook, analyze data, prepare summaries of ongoing experiments. Assist Laboratory Manager with laboratory compliance issues such as IBC, EH&S, Fire Safety, and UCAR inspections.

10% Other responsibilities and special projects assigned as needed, for grant or paper submission. Assist with Center-wide projects as needed.

5% Provide coverage when needed to independently assist with laboratory maintenance and cleanliness as required; wash all glassware generated from laboratory experiments; autoclave glassware, lab solutions, lab supplies, and surgical instruments; clean tissue culture rooms following all established protocols; Provide general maintenance on laboratory equipment including troubleshooting, repairs and cleaning.

5% Prepare common reagents (PBS, TAE, 70% ETOH), agar plates, and broth for general laboratory use in experiments. Using Excel, manage computerized inventory, MSDS, and data sheet records.

  • Other duties as assigned

MINIMUM REQUIREMENTS:

Associates degree (BS preferred) in appropriate discipline plus 4-5 years specialized experience in related field or equivalent combination of education and experience. The ability to work in a diverse lab team environment highly preferred.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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