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University of Rochester Laboratory Technician IV (S) - 228011 in Rochester, New York

Laboratory Technician IV (S) Job ID 228011

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 079 Cancer Center M&D

Responsibilities

POSITION SUMMARY:

To provide research support by independent or semi-supervised coordination and execution of experiments to establish the function and therapeutic targeting of various oncogenes of interest in ovarian cancer to the principal investigators Drs. Richard Moore and Rachael Turner in the Wilmot Cancer Institute. The goals of this project are to decipher the mechanism of action of small molecule therapies developed in the laboratory for the treatment of ovarian cancer. This individual is expected to conduct in vitro and animal experiments and process/use human or animal tissue samples to design and conduct experiments that should support publication of manuscripts in scientific journals and grant proposals in timely manner. This position requires that the individual be able to conduct, troubleshoot and assist in redesigning experiments as needed.

SUPERVISION:

This position reports to Richard Moore, MD and Rachael Turner, MD

TYPICAL ASSIGNMENTS AND RESPONSIBILITIES:

  • 25% Perform cell culture and manipulation of human cell lines including cloning for in vivo and in vitro experiments.

  • 15% Assist in the design of polychromatic cell sorting and flow cytometry panels. Compensate and assist in analyzing flow cytometry data and perform flow cytometry analysis. Prepare cells by staining for flow cytometry analysis, multi-parameter flow cytometry and cell sorting, using scientific techniques such as lymphocyte assays, cell sorting and immunohistochemistry.

  • 15% Animal experiments, breeding and maintenance of HE4 knockout mice under development. Xenografting tumors in immune deficient mice, drug treatment, tumor growth monitoring and tumor harvesting post sacrifice.

  • 15% Assist with general laboratory assays including, western blots, cell culture, ELISA and circulating tumor cell capture on the Parasortix platform.

  • 10% Data analysis and interpretation.

GENERAL LAB RESPONSIBILITIES:

  • 10% Participate in regular lab meetings including presentation of results. Develop figures and write methodologies for results to be published or included in the grant applications.

  • 10% Oversight responsibility of lab supplies, safety compliance, overseeing the utilization/maintenance of various instrumentations and lab equipment.

EDUCATIONAL AND SKILL SET REQUIREMENTS:

Associate's degree in appropriate discipline plus 4-5 years specialized experience in related field, or equivalent combination of education and experience required. BS or equivalent degree in biological sciences with 3-4 years of laboratory experience preferred. Expertise with animal experiments, breeding, colony maintenance, xenograft implantation in nude or NSG mice, rats, multispectral imaging flow cytometry, microscopy and molecular biology techniques including cloning and PCR are essential. Excellent oral and written communication skills. Ability to work in a collaborative work environment. Must be able to work with rodents and must complete human subjects research training.

Other desired skills: Proficiency in Prism Graph Pad, MS Word, Excel, Photoshop, PowerPoint, FlowJo, and statistical software. Occasional weekend and evening hours may be necessary.

NOTE: This document describes typical duties and responsibilities and is not intended to limit management from assigning other work as required.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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