Laboratory Technician IV (S)
In accordance with broadly-outlined objectives, requirements and approaches, the technician plans and conducts technical procedures, non-routine and complex in nature. The technician will lead translational research activities involving imaging and staining of immunohistochemistry samples, flow cytometry, genotyping, and other laboratory techniques. Their focus will be on the molecular mechanisms of experimental arthritis, skin fibrosis, vascular dysfunction, and rheumatoid arthritis / scleroderma pathogenesis, under the direct supervision of the PI and/or Sr. Researchers
JOB DUTIES AND RESPONSIBILITIES:
Histology. Plans and performs experiments requiring a high degree of skill and training on immunohistochemistry, immunostaining, imaging and tissue histology study. Utilizes laboratory equipment including fluorescence microscope, slide scanners, micropipettes, and oven. Will assist with acquiring samples and processing sections as needed. Keep track of and organize histology samples. Produces data tables and figures for research papers and presentation and reviews the draft material for content, organization, clarity, grammar and style.
Flow cytometry. Plan and execute sample collection, preparation, and flow cytometry following provided protocol. Make recommendations regarding Modifying/revising protocol as needed with Sr. Researcher. Utilize flow cytometry core facility for multi-parameter flow cytometric data collection. Performs moderate to complex analysis and calculation of data; interprets and makes recommendations. Produces data tables and figures for research papers and presentation and reviews the draft material for content, organization, clarity, grammar and style.
Genotyping. Plan and perform genotyping of the animal colonies. Genotyping will be done by using techniques requiring a high degree of skill and training such as tissue digestion, PCR, gel electrophoresis, cryo-embedding of tissue samples, and fluorescence imaging of histology slides. Communicate with either the PI or Sr. Researcher in the lab the results. Help with managing the animal colony as needed.
In vitro work. Help with cell culture by changing media, passaging cells, and checking cell culture equipment operation. Let Sr. Researcher know if any of the cell culture equipment is not operating properly.
Lab maintenance and Equipment. Keep track of lab supplies and inform the lab manager when in need of ordering more supplies. Autoclave lab items as needed. General lab upkeep. Ensure compliance with Environmental Health and Safety and Training for Research Laboratory Personnel. Oversee equipment maintenance (including centrifuges, incubators, tissue culture hoods, PCR machines, plate reader, and micropipettes) and train individuals in use of equipment.
Provides technical oversight to lower-level research students. Coordinates the training and performance of lower-level students. Inspects completed work and assures quality and compliance of standards and specifications. Educates on the monitoring and sampling of equipment; may train staff and identify technical training needs
Works closely with Principal Investigator and other research staff to modify or develop new procedures and policies. May monitor a lab budget, working closely with division administrator and senior accountant and attending monthly ledger review meetings
Other duties as assigned. E.g. daily monitoring of experimental mice.
High School diploma or GED required
Associates degree in Health Science, Biology, Chemistry or other science related field of study preferred
5 years of experience with performing basic sampling, monitoring or research activities; compiling and collecting data and preparing reports required.
or equivalent combination of education and experience required
Excellent organizational, interpersonal and communication skills, including written communication preferred.
Previous experience with animal work, histology, flow cytometry, and/or biochemistry is preferred
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How To Apply
All applicants must apply online.
EOE Minorities/Females/Protected Veterans/Disabled
Pay Range: $18.89 - $26.44 Hourly
The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job’s compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.
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Location: School of Medicine & Dentistry
Full/Part Time: Full-Time