Research Technician II

Rochester, NY

GENERAL PURPOSE:

This individual will identify and validate gut microbiota metabolites that determine the phagocytic functions of innate immune cells (macrophage and neutrophils) isolated from a Type 2 diabetic mouse model and human subjects with Type 2 diabetes. It is expected that the candidate will maintain a comprehensive laboratory notebook, present data at project team meetings, and develop independence over time in performing and designing the experiments.

JOB DUTIES AND RESPONSIBILITIES:

  • Culture and isolate neutrophils and macrophage from mouse bone marrow or human serum.

  • Carry out phagocytosis assays to determine the impact of identified metabolites on neutrophil/macrophage killing of Staphylococcus aureus .

  • RNA extraction and qRT-PCR to monitor expression of cytokines and other immune markers.

  • Assists in developing, devising or modifying methods and procedures and their implementation.

  • Participate in project related meetings, presenting data and results.

  • Maintain lab safety and cleanliness.

  • Cleans and performs routine maintenance on equipment.

  • Performs general laboratory duties such as preparing reagents, restocking supplies and ordering.

Other duties as assigned

QUALIFICATIONS:

  • High school diploma or GED required.

  • Bachelor’s degree in appropriate discipline. Previous instruction in theoretical aspects of cell and molecular biology preferred.

  • 1 year performing basic sampling, monitoring or research activities, compiling and collecting data and preparing reports or equivalent combination of education and experience required.

  • Knowledge of OSHA safety requirement and scientific methods required.

  • Previous experience with basic eukaryotic and bacterial cell culture techniques, RNA and DNA extraction and qRT-PCR and microscopy is highly desirable.

  • Ability to perform mathematical calculations using algebra and practically apply percentages, fractions, and ratios preferred.

  • Strong attention to detail and ability to perform experiments with precision preferred.

  • Ability to communicate effectively (including written communication); follow instructions exactly, and record informationaccurately preferred.

The University of Rochester is committed to fostering, cultivating, and preserving a culture of equity, diversity, and inclusion to advance the University’s mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other status protected by law. This commitment extends to the administration of our policies, admissions, employment, access, and recruitment of candidates from underrepresented populations, veterans, and persons with disabilities consistent with these values and government contractor Affirmative Action obligations.

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

Pay Range

Pay Range: $ 19.37 - $ 27.12 Hourly

The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job’s compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations.

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Location: School of Medicine & Dentistry
Full/Part Time: Full-Time
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