University of Rochester Technical Assoc I (S) - 223252 in Rochester, New York
Technical Assoc I (S)
School of Medicine & Dentistry
Full Time 40 hours Grade 051 Pediatrics M&D Inf Diseases
8:00 AM - 4:30 PM
This research position is for a lab manager in the lab of Dr. Regina Rowe in the Department of Pediatrics, Division of Infectious Diseases. The lab focuses on how allergic diseases, such as asthma, eczema, and allergic rhinitis, impact antiviral responses by innate immune cells, T cells, and respiratory epithelial cells. Responsibilities will include but are not limited to: ordering lab materials and reagents, organization of the laboratory, assist PI in writing of SOPs and protocols, purification of primary immune cells, culturing primary immune and respiratory epithelial cells, media preparation and cell culture, virus propagation and cell infections, preparation and staining of cells for flow cytometry, and molecular and cell biology techniques as indicated for given experiments. The candidate will work independently, organize and troubleshoot experiments, and train other lab personnel to the best of her/his/their ability under the guidance of the PI. Participation in regularly scheduled meetings, and attendance and presenting at lab meeting or other conferences are required.
This position reports to Regina Rowe, M.D. Ph.D., Assistant Professor of Pediatrics
TYPICAL ASSIGNMENTS AND RESPONSIBILITIES:
Under general guidance and with latitude for exercising independent technical judgment, provides technical support and execution of experiments to better understand the impact of allergy on antiviral immune responses. Specific duties may include but are not limited to:
Works independently with only occasional suggestions in modifying, varying or adapting standard laboratory procedures to isolate primary immune and airway cells from blood and tissues (human and murine).
Performs cell culture of primary cells and cell lines including virus infections.
Prepares cells and staining for flow cytometry analysis. Compensates and analyzes imaging flow cytometry data.
Other molecular and cell biology techniques: Enzyme-linked immunoassays (ELISA), western blotting, isolation of RNA and DNA, and PCR/RT-PCR/qPCR/qRT-PCR.
General lab maintenance including ordering and organization, cataloging of clinical samples, and writing of SOPs and protocols with PI guidance. Other duties as assigned.
As directed by the PI, teaches laboratory techniques and instrumentation procedures to students and laboratory technical support staff; and counsels them on technical problems.
Participates in regular lab meetings, including presentations and regular (weekly) meetings with Dr. Rowe. Keeps abreast of trends in field of interest by reading professional journals and attending meetings and seminars,
Searches literature for reference to technical problems involved in research projects, sometimes including literature in foreign languages; writes procedures; prepares charts, graphs and tables; and assists in the preparation of materials for publication.
Bachelor’s degree and one year of experience in the biological sciences is preferred. Prior laboratory experience and familiarity with general biological techniques is required with experience in microbiology or immunology being preferred. Specific experience making solutions/buffers/media, sterile techniques, cell or bacterial culture methods, and molecular biology techniques is desired. Candidate must have excellent command of oral and written English language, strong interpersonal skills, and be able to work in a collaborative team environment. Computer skills including MS Word, Excel, and PowerPoint are necessary. Candidate should be highly organized and detail/documentation oriented.
Laboratory duties will require working in a modern biomedical research facility in the vicinity of properly contained chemical, biological and radiological materials.
Safe handling of infectious agents including human blood will be required.
All procedures and facilities conform to federal, local and institutional safety and ethical guidelines.
Full time, 40 hours/week. Standard daytime hours are expected but will vary depending on needs of the lab. Occasional night and weekend hours may be required.
How To Apply
All applicants must apply online.
EOE Minorities/Females/Protected Veterans/Disabled