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University of Rochester Technical Assoc I (S) - 231786 in Rochester, New York

Technical Assoc I (S) Job ID 231786

Location School of Medicine & Dentistry Full/Part Time Full-Time Favorite Job Regular/Temporary Regular Opening

Full Time 40 hours Grade 051 Neurology/Ctr & Gene Ther

Schedule

M-F; Some WKNDS

Responsibilities

GENERAL PURPOSE:

Provides direct research support at the professional entry level in a large Neuroscience laboratory. Is responsible for conducting and analyzing a set of experiments that comprise a coherent part of a larger project; and researching and resolving technical problems within the scope of those experiments.

TYPICAL ASSIGNMENTS AND RESPONSIBILITIES:

Under general guidance and with latitude for exercising independent technical judgment, candidate will work independently with only occasional suggestions to perform the following research activities, which are limited in scope and complexity:

  1. Supports the Principal Investigator(s) in the creation of CRISPR Knock-in cell lines. Specifically, the role will perform a wide range of complex molecular and cellular biology procedures encompassing nucleic acid, and protein analyses from tissue and cell cultures. (30%)

  2. Specific techniques may include, but are not limited to, isolation of DNA, RNA, and proteins from tissue and cell culture; immunochemistry and immunofluorescence, virus titration and purification, antibody ELISA, PCR and RT¿PCR; real¿time PCR, and cloning. (25%)

  3. Performs specialized surgical operations on laboratory animals for the collection of scientific data: performs complex brain surgery on mice, using aseptic stereotactic technique. (10%)

  4. Responsible for animal colony maintenance and documentation, including the reproduction and maintenance of multiple, scientifically discrete transgenic and mutant animal colonies. Manages complex data bases of mouse colony matings and lineages and reproductive data. Genotypes mice using Polymerase Chain Reaction (PCR) techniques. Verifies colony census and manages records of Department of Lab Animal Medicine (DLAM) procedures. Acts as liaison between vivarium and lab on animal colony matters. Teaches and supervises students and other lab staff who genotype mice or work in mouse colonies. Advises students and other lab staff on technical problems. (10%)

  5. Prepares progress reports on project, consisting of photomicrographs and statistical analyses of cell counts as relevant. (10%).

  6. Searches literature for reference to technical problems involved in research projects, writes procedures; prepares charts, graphs and tables; and assists in the preparation of materials for publication. Abstracts relevant scientific articles of value to the prosecution of research problems and adapts techniques and makes recommendations to meet research project goals. 5%

  7. Maintains laboratory equipment and works with staff to ensure compliance with university and federal laboratory animal and safety regulations. 5%

  8. Other projects and job duties as assigned. 5%

REQUIREMENTS:• Bachelors degree in biological discipline and 1 year of related work experience involving direct assistance to researchers; or any equivalent combination of experience, training and education.. • Applicants should be organized, self-motivated and capable of working independently with only occasional suggestions in modifying, varying or adapting standard laboratory procedures. • Possess strong interpersonal and communication skills (including written communication) with the ability to readily learn new experimental techniques. • Strong attention to detail, analytical, organizational and computer

How To Apply

All applicants must apply online.

EOE Minorities/Females/Protected Veterans/Disabled

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